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上海起發(fā)arthusbio2022年產(chǎn)品目錄

更新時間：2022-07-25 點擊次數(shù)：1765

S-腺苷甲硫氨酸（SAM-e，SAMe，SAM，AdoMet，ademetionine）由GL Cantoni于1952年在意大利發(fā)現(xiàn)。它是由三磷酸腺苷（ATP）和甲硫氨酸經(jīng)甲硫氨酸腺苷轉(zhuǎn)移酶制成的。S-腺苷甲硫氨酸是大多數(shù)生物體中的關(guān)鍵代謝物。它通過脫羧作用發(fā)揮重要的轉(zhuǎn)甲基、轉(zhuǎn)硫和氨丙基化功能。
由于 SAM 是一種本質(zhì)上不穩(wěn)定的分子，其 258-260 nm 的最大光密度不是顯著的吸收，因此測定其在各種生物體液和組織中的濃度一直是一項具有挑戰(zhàn)性的任務(wù)。一種不需要昂貴儀器（LC、MS 和組合）的簡單、方便的方法顯然是確定 SAM 的生物濃度以及監(jiān)測其在體液、組織和細(xì)胞器中的變化和代謝路徑的理想選擇。

據(jù)稱 SAM-e 有助于以下疾?。阂钟舭Y、帕金森病、骨關(guān)節(jié)炎癥狀、肝病、肝內(nèi)膽汁淤積癥（尤其是妊娠期）、纖維肌痛、艾滋病相關(guān)的脊髓病（與艾滋病相關(guān)的脊柱和神經(jīng)疾?。?。也有有限的研究參考了 SAMe 在以下情況中的作用：注意力缺陷多動障礙 (ADHD)、經(jīng)前期綜合征 (PMS)、經(jīng)前期煩躁癥 (PMDD)、慢性疲勞綜合征 (CFS)、心臟病、智力缺陷表現(xiàn)、多發(fā)性硬化、癡呆、阿爾茨海默病、肌腱炎、滑囊炎、脊髓損傷、癲癇發(fā)作、偏頭痛、慢性腰痛、鉛中毒和衰老。
如今，當(dāng) SAM 每天用作營養(yǎng)補充劑時，了解其影響和后果也是至關(guān)重要的。它已被廣泛用作非處方藥，以緩解抑郁癥、骨關(guān)節(jié)炎等系統(tǒng)。

甲基化

甲基化幾乎涉及生活的方方面面。SAM 是 DNA、RNA、蛋白質(zhì)、脂質(zhì)、磷脂、激素和神經(jīng)遞質(zhì)中各種甲基轉(zhuǎn)移反應(yīng)的甲基供體。適當(dāng)?shù)?DNA 甲基化對于正常的胚胎發(fā)育至關(guān)重要。然而，當(dāng)甲基轉(zhuǎn)移酶基因純合缺失時，它是致命的。

已在許多腫瘤中發(fā)現(xiàn)不正確甲基化的 DNA。DNA甲基化模式的改變會誘導(dǎo)癌基因的表達(dá)或抑制腫瘤抑制基因的表達(dá)。缺乏甲基的飲食會導(dǎo)致嚙齒動物患肝癌。

重要過程

DNA 甲基化導(dǎo)致基因組免受限制性內(nèi)切酶的影響。SAM 通過與嵌入某些 mRNA 的非編碼區(qū)內(nèi)的結(jié)構(gòu)域結(jié)合來控制基因表達(dá)。SAM 在基本生物分子的生產(chǎn)中提供甲基，包括：

乙酰左旋肉堿：神經(jīng)營養(yǎng)素和膜轉(zhuǎn)運劑
腎上腺素和腎上腺素：內(nèi)源性兒茶酚胺、壓力激素和神經(jīng)遞質(zhì)
磷脂酰膽堿：最重要的膜磷脂
肉堿：脂肪燃燒器
磷酸肌酸：主要的 ATP 水庫
褪黑激素：晝夜節(jié)律調(diào)節(jié)劑

SAM 是細(xì)胞生長和修復(fù)所必需的。它還參與幾種影響情緒的激素和神經(jīng)遞質(zhì)的生物合成，例如腎上腺素。甲基轉(zhuǎn)移酶還負(fù)責(zé)將甲基添加到信使 RNA 中靠近 5' 帽的第一個和第二個核苷酸的 2' 羥基上

轉(zhuǎn)硫

轉(zhuǎn)硫開始于 S-腺苷高半胱氨酸或 SAH，它是 S-腺苷甲硫氨酸 (SAM) 在將甲基轉(zhuǎn)移出 SAM 后的殘留結(jié)構(gòu)。SAH 的水解產(chǎn)生同型半胱氨酸。這會轉(zhuǎn)化為胱硫醚、半胱氨酸，最后轉(zhuǎn)化為谷胱甘肽，即肝細(xì)胞抗氧化劑和拯救生命的解毒劑。由于膳食半胱氨酸含量低，高達(dá) 80% 的膳食半胱氨酸通過胃腸道失去其巰基，SAM 是半胱氨酸的主要來源，半胱氨酸是谷胱甘肽的組成部分。作為一種抗氧化劑，它可以保護(hù)身體免受體內(nèi)活性氧分子的破壞。這些活性氧分子可能來自體內(nèi)或環(huán)境污染，被認(rèn)為在衰老過程和退行性疾病的發(fā)展中發(fā)揮作用。一般來說，

氨基丙基化

氨丙基化是SAM通過脫羧（脫羧酶）引發(fā)的另一個過程。脫羧后的 SAM 然后與腐胺偶聯(lián)生成亞精胺和精胺，這些亞精胺和精胺對細(xì)胞生長、分化以及 DNA 和 RNA 的穩(wěn)定性至關(guān)重要。此外，多胺合成的副產(chǎn)物甲基硫腺苷 (MTA) 是一種強大的鎮(zhèn)痛和抗炎劑。這可能至少部分地歸因于在使用 SAMe 治療骨關(guān)節(jié)炎、類風(fēng)濕性關(guān)節(jié)炎和纖維肌痛中觀察到的臨床益處。

Arthus Biosystems 很高興提供一系列研究試劑和試劑盒，以幫助開展與蛋氨酸循環(huán)相關(guān)的科學(xué)研究，蛋氨酸循環(huán)是所有生物體進(jìn)化過程中保守的關(guān)鍵生化途徑。我們的 SAM 和 SAH 單克隆抗體具有非常高的滴度和親和力，讓您只需一瓶即可完成大部分研究。迄今為止，我們的抗 SAM 和 SAH 抗體均具有*佳靈敏度。我們還提供經(jīng)過驗證的 SAM 和 SAH 偶聯(lián)物以及生物s等示蹤分子，以幫助您研究生物分子-生物分子的相互作用并開發(fā)進(jìn)一步的檢測方法。

上海起發(fā)arthusbio2022年產(chǎn)品目錄

Catalog No.	Name	Description	Packaging
Immunoassay Kits and Strips
IK00201	SAM ELISA	S-adenosylmethionine ELISA kit	96 tests
IK00201s	SAM ELISA s	S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples	96 tests
IK00202	SAM ELISA 2	S-adenosylmethionine ELISA kit, broad range	96 tests
IK00202s	SAM ELISA 2s	S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range	96 tests
IK00203	SAM ELISA 3	S-adenosylmethionine ELISA kit, broad range	48 tests
IK00203s	SAM ELISA 3s	S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range	48 tests
IK00204	SAM ELISA 4	S-adenosylmethionine ELISA kit for cell culture supernatant or other samples	96 tests
IK00301	SAH ELISA	S-adenosylhomocysteine ELISA kit	96 tests
IK00301s	SAH ELISA s	S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples	96 tests
IK00302	SAH ELISA 2	S-adenosylhomocysteine ELISA kit, broad range	96 tests
IK00302s	SAH ELISA 2s	S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range	96 tests
IK00303	SAH ELISA 3	S-adenosylhomocysteine ELISA kit, broad range	48 tests
IK00303s	SAH ELISA 3s	S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range	48 tests
IK00304	SAH ELISA 4	S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples	96 tests
IK00401	MAT Activity Assay	Methionine Adenosyltransferase (MAT) activity assay kit	96 tests
IK00403	MAT Activity Assay 3	Methionine Adenosyltransferase (MAT) activity assay kit	48 tests
Antibodies and Conjugates
MA00201-50	Mouse anti-SAM 1b	Mouse monoclonal antibody to S- denosylmethionine Clone 118-6	100µg
MA00201-100	Mouse anti-SAM 1a	Mouse monoclonal antibody to S-Adenosylmethionine Clone 118-6	50µg
MA00202-50	Mouse anti-SAM 2a	Mouse monoclonal antibody to S- denosylmethionine Clone 84-3	50µg
MA00202-100	Mouse anti-SAM 2b	Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-3	100µg
MA00203-50	Mouse anti-SAM 3a	Mouse monoclonal antibody to S-Adenosylmethionine Clone 118-18	50µg
MA00204-50	Mouse anti-SAM 4a	Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-19	50µg
PA00201-50	Rabbit anti-SAM a	Rabbit polyclonal antibody to S-Adenosylmethionine	50µg
PA00201-100	Rabbit anti-SAM b	Rabbit polyclonal antibody to S-Adenosylmethionine	100µg
MA00301-50	Mouse anti-SAH 1a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-1	50µg
MA00302-50	Mouse anti-SAH 2a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-10	50µg
MA00303-50	Mouse anti-SAH 3a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-3	50µg
MA00304-50	Mouse anti-SAH 4a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-16	50µg
MA00305-50	Mouse anti-SAH 5a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-1	50µg
MA00306-50	Mouse anti-SAH 6a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-2	50µg
MA00307-50	Mouse anti-SAH 7a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 839-6	50µg
MA00308-50	Mouse anti-SAH 8a	Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 839-12	50µg
MAF00201-25	AF6-anti-SAM 1a	Alexa Fluor® 647 (AF6) conjugated anti-SAM monoclonal antibody Clone 118-6	25µg
MAF00201-50	AF6-anti-SAM 1b	Alexa Fluor® 647 (AF6) conjugated anti-SAM monoclonal antibody Clone 118-6	50µg
MAF00202-50	AF6-anti-SAM 2a	Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3	50µg
MAF00301-25	AF488-anti-SAH 1a	Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 301-3	25µg
MAF00301-50	AF488-anti-SAH 1b	Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 301-3	50µg
MAF00302-50	AF488-anti-SAH 2a	Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 839-6	50µg
MAH00201-50	HRP-anti-SAM 1a	Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 118-6	50µl
MAH00202-50	HRP-anti-SAM 2a	Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 84-3	50µl
MAH00203-50	HRP-anti-SAM 3a	Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 118-18	50µl
MAH00204-50	HRP-anti-SAM 4a	Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 84-19	50µl
MAH00301-50	HRP-anti-SAH 1a	Horseradish peroxidase (HRP) conjugated anti-SAH monoclonal antibody Clone 301-3	50µl
MAH00302-50	HRP-anti-SAH 2a	Horseradish peroxidase (HRP) conjugated anti-SAH monoclonal antibody Clone 301-16	50µl
MAH00701-50	HRP-anti-DIG	Peroxidase-conjugated IgG Fraction Monoclonal Mouse Anti-Digoxigenin	50µl
PA00401-50	Rabbit anti-MAT s	Rabbit polyclonal antiserum against methionine adenosyltransferase	50µl
PA00401-50	Rabbit anti-MAT g	Rabbit polyclonal antiserum against methionine adenosyltransferase (with glycerol)	50µl
Compounds and Conjugates
AST00201	Aza-SAM	A series of S-adenosylmethionine analog Aza-SAM standards: 500nM, 250nM, 125nM, 62.5nM, 31.25nM, 15.625nM, 7.8125nM, 0nM	1ml
AST00201-1	Aza-SAM a	S-adenosylmethionine analog Aza-SAM standard. Buffer (4 ml) for serial dilution may be supplied (additional $15 each).	1ml (500nM)
AST00201-200	Aza-SAM b	S-adenosylmethionine analog Aza-SAM standard. Buffer (10 ml) for serial dilution may be supplied (additional $15 each).	200ul (5µM)
AST00201-1s	Aza-SAM as	S-adenosylmethionine analog Aza-SAM standard for serum/plasma. Buffer (3.2 ml) for serial dilution may be supplied.	1ml (1µM)
ACT00201-5	PLL-aza-SAM a	Poly-lysine was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). Coating for 10-20 96-well micro-titer plates.	5µg
ACT00201-10	PLL-aza-SAM b	Poly-lysine was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). Coating for 20-40 96-well micro-titer plates.	10µg
ACT00202-50	Bio-SAM a	Biotin is conjugated to NH2 group of S-adenosylmethionine.	50µg
ACT00202-100	Bio-SAM b	Biotin is conjugated to NH2 group of S-adenosylmethionine.	100µg
ACT00203-50	Bio-6C-SAM 1a	Biotin is conjugated to NH2 of SAM through 6-aminohexanoic acid.	50µg
ACT00203-100	Bio-6C-SAM 1b	Biotin is conjugated to NH2 of SAM through 6-aminohexanoic acid.	100µg
ACT00204-5	BSA-aza-SAM a	Bovine serum Albumin was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). Coating for 10-20 96-well micro-titer plates.	5µg
ACT00205-10	Bio-6C-aza-SAM 1a	Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid.	10µg
ACT00205-50	Bio-6C-aza-SAM 1b	Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid.	50µg
ACT00205-100	Bio-6C-aza-SAM 1c	Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid.	100µg
ACT00206-10	Bio-12CN-aza-SAM 1a	Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker.	10µg
ACT00206-50	Bio-12CN-aza-SAM 1b	Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker.	50µg
ACT00206-100	Bio-12CN-aza-SAM 1c	Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker.	100µg
AST00301-1	SAH-Na	S-Adenosylhomocysteine standard ( SAH Sodium). Buffer (18 ml) for serial dilution may be supplied (additional $15 each).	1ml (4µM)
AST00301-1s	SAH-Na s	S-Adenosylhomocysteine standard ( SAH Sodium) for serum/plasma. Buffer (8ml) for serial dilution may be supplied (additional $60 each).	0.5ml (4µM)
ACT00301-50	BSA-SAH	BSA was conjugated to S-Adenosylhomocysteine. Coating for 10 96-well micro-titer plates.	50µg (20µg)
ACT00302-50	Bio-SAH a	Biotin is conjugated to NH2 group of S-adenosylhomocysteine.	50µg
ACT00302-100	Bio-SAH b	Biotin is conjugated to NH2 group of S-adenosylhomocysteine.	100µg
ACT00303-50	Bio-6C-SAH 1a	Biotin is conjugated to NH2 of SAH through 6-aminohexanoic acid.	50µg
ACT00303-100	Bio-6C-SAH 1b	Biotin is conjugated to NH2 of SAH through 6-aminohexanoic acid.	100µg
ACT00304-25	Dig-6C-SAH 1a	Digoxin is conjugated to NH2- of SAH through 6-bromocaproic acid	25µg
ACT00304-50	Dig-6C-SAH 1b	Digoxin is conjugated to NH2 of SAH through 6-bromocaproic acid.	50µg
ACT00304-100	Dig-6C-SAH 1c	Digoxin is conjugated to NH2 of SAH through 6-bromocaproic acid.	100µg
ACT00305-10	Bio-12CN-SAH 1a	Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker.	10µg
ACT00305-50	Bio-12CN-SAH 1b	Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker.	50µg
ACT00305-100	Bio-12CN-SAH 1c	Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker.	100µg
AST00401-50	MAT a	Recombinant methionine adenosyltransferase (E.C. 2.5.1.6), E. Coli expression	50µg
AST00401-500	MAT b	Recombinant methionine adenosyltransferase (E.C. 2.5.1.6), E. Coli expression	500µg
ACT00501-100	BSA-HCY	Bovine serum albumin (BSA) was conjugated to homocysteine (HCY).	100µg
AST00601-50	HMT a	Recombinant Homocysteine S-methyltransferase (E.C. 2.1.1.10), E. Coli expression	50µg
AST00601-500	HMT b	Recombinant Homocysteine S-methyltransferase (E.C. 2.1.1.10), E. Coli expression	500µg

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