pTK-hyg

Description

pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the 

selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable 

cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an 

enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived 

plasmids upon cointegration into the host cell's genome.

Use

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains 

a gene of interest using any standard transfection technique. Most mammalian cell lines 

require 200 µg/ml of Hygromycin to select for stably transformed cells.

Location of Features

• HSV TK polyA signals: 1473–1478 & 1486–1491 （complementary）

• Hygr

 （hygromycin resistance gene）: 1537–2574 （complementary）

• P

HSV TK （HSV TK promoter）: 2588–2835 （complementary）

• pUC origin ofreplication: 3152–3795

• Ampr

 （ampicillin resistance gene; beta-lactamase）: 3943–4803

Propagation in E. coli

• Suitable host strains: DH™ and other general purpose strains. 

• Selectable marker: plasmid confers resistance to ampicillin （50 µg/ml） on E. coli hosts.

• E. coli replication origin: pUC