The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin （pCoBlast） or hygromycin （pCoHygro） selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
Small size （5.4 kb） to improve DNA yields and increase subcloning efficiency.
C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody.
C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin.

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.