pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors （Figure 1） offer the following features:

• Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins （Figure 2）

• Cytomegalovirus （CMV） promoter for high-level constitutive expression

• C-terminal polyhistidine （6xHis） tag for rapid purification with nickel-chelating resin and detection with an Anti-His（C-term） Antibody

• C-terminal c-myc epitope for detection with an Anti-myc Antibody

• Bovine Growth Hormone （BGH） polyadenylation signal and transcription termination sequence to enhance mRNA stability

• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen （e.g., COS-1, COS-7）

The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.