lifediagnostics

鏈接：https://lifediagnostics.com

Life Diagnostics生產(chǎn)ELISA試劑盒、SPARCL™試劑盒、用于臨床前研究和獸醫(yī)診斷的純化生物標(biāo)志物和抗體。Life Diagnostics的專業(yè)領(lǐng)域包括心血管、急性期、免疫毒性和免疫學(xué)生物標(biāo)志物，涉及的物種包括老鼠、大鼠、兔子、貓、狗、豬、牛、猴子和雞。此外，Life Diagnostics還開發(fā)了抗PEG單克隆抗體和ELISA試劑盒，用于檢測PEG、mPEG化蛋白和抗PEG抗體。Life Diagnostics公司一直致力于為客戶提供高質(zhì)量的產(chǎn)品和優(yōu)質(zhì)的服務(wù)，贏得了廣泛的贊譽和信任。

主要產(chǎn)品線

一、SPARCL™試劑盒

SPARCL™（空間近距分析試劑捕獲發(fā)光）檢測使用兩種特定的抗體；一種與HRP結(jié)合，另一種與二氫化吖啶結(jié)合，一種化學(xué)發(fā)光底物。當(dāng)HRP和二氫化吖啶結(jié)合的抗體與它們的靶生物標(biāo)志物結(jié)合時，加入過氧化氫（觸發(fā)溶液）后，HRP催化氧化鄰近二氫化吖啶分子，導(dǎo)致與生物標(biāo)志物濃度成比例的化學(xué)發(fā)光。遠(yuǎn)離HRP的二氫化吖啶分子不被氧化，因此不產(chǎn)生發(fā)光。這一原理可以開發(fā)一系列快速敏感的均相免疫分析方法。

典型的SPARCL™檢測不需要洗滌步驟，只需一次孵育，從開始到結(jié)束大約需要45分鐘。而ELISA需要1-4次孵育，1-3次洗滌步驟，需要2-4+小時。SPARCL™試劑盒需要一個能夠同時加樣和測量的光度計。Life Diagnostics提供一系列的SPARCL™檢測試劑盒。

SPARCL™—ELISA的優(yōu)秀替代品

–簡單發(fā)光免疫測定

–單次培養(yǎng)30分鐘

–無清洗步驟

–高靈敏度

–高吞吐量

參考文獻(xiàn)

1.Baka R, et al. Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic sezures. J Proteomics 231:1039777 (2021)

2.Kules J, et al. The plasma proteome and the acute phase protein response in canine pyometra. J Proteomics 223:103817 (2020)

3.Liu M, et al. Cardiovascular-renal axis disorder and the acute-phase proteins in cats with congestive heart failure caused by primary cardiomyopathy. J Vet Intern Med. 34(3):1078-1090 (2020)

4.Dalanezi FM, et al. Concentrations of acute phase proteins in milk from cows with clinical mastitis caused by different pathogens. Pathogens 9(9):706 (2020)

5.Boulay I, et.al. SPARCL™: Use of a novel technology in validation of a Non-human primate and rat cardiac troponin-I assay in serum. CiToxLAB, 2016

6.Boulay I, et.al. SPARCL™: Use of a Novel Technology in Validation of a Non-Human Primate C-Reactive Protein Assay in Serum. CiToxLAB. 2016

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二、ELISA試劑盒

抗KLH、抗SRBC、抗破傷風(fēng)毒素、抗DNP和抗TNP的IgM和IgG被用作免疫毒性的生物標(biāo)志物。Life Diagnostics為小鼠、大鼠、雞、猴子和人類生產(chǎn)這些生物標(biāo)志物的ELISA試劑盒。

使用Life Diagnostics ELISA試劑盒發(fā)表的部分文獻(xiàn)

1.Lo J, et al. Immature dendritic cell therapy confers durable immune modulation in an antigen-dependent and antigen-independent manner in nonobese diabetic mice. Journal of Immunology Research. Volume 2018 Article ID 5463879 (2108) [Mouse anti-KLH IgG & IgM]

2.Ksiazek A and Konarzewski M. Heat dissipation does not suppress an immune response in laboratory mice divergently selected for basal metabolic rate (BMR). Journal of Experimental Biology. 219:1542-1551 (2016) [Mouse anti-KLH IgG & IgM]

3.Tan X, et al. Immunotoxicological evaluation of genetically modified rice expressing Cry1Ab/AcProtein (TT51-1) by a 6-month feeding study on cynomolgus monkeys. PLoSONE 11(9):e0163879.doi:10.1371/journal.pone.0163879 (2016) [Monkey anti-KLH IgG & IgM]

4.Ma H, Wan S, and Xia C-Q. Immunosuppressive CD11b+Ly6Chi monocytes in pristane-induced lupus mouse model. Journal of Leukocyte Biology 99:1121 (2016). [Mouse anti-KLH IgG]

5.Nixon AE, et al. Fully human monoclonal antibody inhibitors of the neonatal Fc receptor reduce circulating IgG in non-human primates. Frontiers in Immunology. Volume 6, Article 176. (2015) [Monkey anti-tetanus toxoid & anti-KLH IgG & IgM]

6.Bernett MJ, et al. Immune suppression in cynomolgus monkeys by XPro9523. (2013) https://doi.org/10.4161/mabs.23976 [Monkey anti-KLH IgG & IgM]

7.Nain S and Smits JEG. Pathological, immunological and biochemical markers of subchronic arsenic toxicity in rats. Environmental Toxicology. (2010) http://onlinelibrary.wiley.com/doi/10.1002/tox.20635/abstract [Rat anti-KLH IgG & IgM]

8.Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH and Taparowsky EJ. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J. Exp. Med. 207(5):933-942 (2011) [Mouse anti-SRBC IgG & IgM] http://jem.rupress.org/content/207/5/933.full.pdf+html

9.Schaffer BS, Grayson MH and Wortham JM, et al. Immune competency of a hairless mouse strain for improved preclinical studies in genetically engineered mice. Mol. Cancer Ther. 9:2354-2364 (2010) [Mouse anti-KLH IgG & IgM] http://mct.aacrjournals.org/content/9/8/2354.full.pdf+html

10.Efanov A, Zanesi N, NazaryanN, Santanam U, Palmarchuck A Croce CM and Pekarsky Y. CD5+CD23+ leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation. Leukemia 24:970-975 (2010) [Mouse anti-SRBC IgM]

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三、蛋白

Life Diagnostics提供alpha-1-acid glycoprotein、CRP、haptoglobin、SAA等急性期生物標(biāo)志物、troponin-I、myoglobin、FABP等心血管生物標(biāo)志物、免疫球蛋白、Haptoglobin、Gc-Globulin肝臟生物標(biāo)志物、Troponin-I骨骼肌生物標(biāo)志物等系列產(chǎn)品，用于臨床前研究研究和診斷分析開發(fā)。

部分產(chǎn)品信息

四、PEG相關(guān)產(chǎn)品

聚乙二醇（PEG）鏈連接到治療性抗體和酶上，可以增加生物制劑的循環(huán)半衰期。Life Diagnostics生產(chǎn)單克隆抗體和ELISA試劑盒，用于檢測PEG和甲氧基-PEG化蛋白。此外，Life Diagnostics還提供用于檢測小鼠、大鼠和猴子體內(nèi)抗PEG IgG和IgM的ELISA試劑盒。

部分產(chǎn)品信息

五、抗體

Life Diagnostics提供alpha-1-acid glycoprotein等急性期生物標(biāo)志物、troponin-I、myoglobin、FABP等心血管生物標(biāo)志物、免疫球蛋白、Haptoglobin、Gc-Globulin肝臟生物標(biāo)志物、Troponin-I骨骼肌生物標(biāo)志物等蛋白抗體以及一系列二抗產(chǎn)品。

部分產(chǎn)品信息

下圖為Life Diagnostics公司授權(quán)上海起發(fā)作為中國區(qū)域代理的授權(quán)書。

Life Diagnostics熱賣產(chǎn)品